Dieter P. Reinhardt
Distinguished James 不良研究所 Professor
Canada Research Chair Tier 1 Laureate in Cell-Matrix Biology
MSc (Kaiserslautern)
PhD (Munich)
The dynamics of cell-extracellular matrix interactions in normal and pathological situations. Identification of disease mechanisms in heritable diseases originating in the extracellular matrix.
The Reinhardt laboratory is interested in components and mechanisms involved in the biogenesis, function and pathological aspects of the extracellular matrix and its interactions with cells. The research themes rank around molecular mechanisms matrix proteins and fibers exert in disorders affecting blood vessels, bone, skin and adipose tissue. We are interested how extracellular matrix proteins instruct cells to differentiate, migrate, and proliferate, and how cells sense normal and pathologically altered extracellular matrix. The lab also focuses on how the extracellular matrix regulates key growth factors of the TGF-beta superfamily, and on deciphering regulatory mechanisms mediated by microRNAs. Methodologically, the lab employs a wide spectrum of techniques ranging from in vitro biochemical and biophysical assays, to cell biology methods with primary cells and transformed cell lines, to light and electron microscopic techniques, to in vivo studies with genetically modified mouse models. The Reinhardt laboratory has published over 160 research articles that are cited more than 11,000 times ().
Keywords:聽Extracellular matrix; cell-matrix interactions; genetic disorders; mutations; protein function; protein assembly; blood vessels, bone, skin, adipose tissue
*Wozny MR, *Nelea V, Siddiqui IFS, Wanga S, de Waard V, #Strauss M, #Reinhardt DP, *Co-first authors contributed equally to this work, #Co-corresponding authors. (2024). Microfibril-associated glycoprotein 4 forms octamers that mediate interactions with elastogenic proteins and cells. Nat Commun 15: 4015.
Dinesh NEH, Rousseau J, Mosher DF, Strauss M, Mui J, #Campeau PM, #Reinhardt DP, #Co-corresponding authors. (2024). Mutations in fibronectin dysregulate chondrogenesis in skeletal dysplasia. Cell Mol Life Sci 81: 419.
Dinesh NEH, Baratang N, Rousseau J, Mohapatra R, Li L, Mahalingam R, Tiedemann K, #Campeau PM, #Reinhardt DP, #co-corresponding authors聽(2024).聽Fibronectin isoforms promote postnatal skeletal development.聽Matrix Biol. 133:听86-102听
Dinesh NEH, #Campeau PM, #Reinhardt DP, #Co-corresponding authors. (2024). The integral role of fibronectin in skeletal morphogenesis and pathogenesis. Matrix Biol 134: 23-29.
Brizio M, Mancini M, Lora M, Joy S, Zhu S, Brilland B, Reinhardt DP, Farge D, Langlais D, Colmegna I. (2024). Cytokine priming enhances the antifibrotic effects of human adipose derived mesenchymal stromal cells conditioned medium. Stem Cell Res Ther 15: 329.
de Wagenaar NP, van den Bersselaar LM, Odijk HJHM, Stefens SJM, Reinhardt DP, Roos-Hesselink JW, Kanaar R, Verhagen JMA, Br眉ggenwirth HT, van de Laar IMBH, van der Pluijm I, Essers J. (2024). Functional analysis of cell lines derived from SMAD3-related Loeys-Dietz syndrome patients provides insights into genotype-phenotype relation. Hum Mol Genet 33: 1090鈥�1104.
Ellis MW, Riaz M, Huang Y, Anderson CW, Hoareau M, Li X, Luo H, Lee S, Park J, Luo J, Batty LD, Huang Q, Lopez CA, Reinhardt DP, Tellides G, Qyang Y. (2024). De novo elastin assembly alleviates development of supravalvular aortic stenosis. Arterioscler Thromb Vasc Biol 44: 1674-1682.
Siddiqui IFS, Muthu ML, Reinhardt DP. (2024). Isolation and adipogenic differentiation of murine mesenchymal stem cells harvested from macrophage-depleted bone marrow and adipose tissue. Adipocyte 13: 2350751.
Zeyer KA, Bornert O, Nelea V, Bao X, Leytens A, Sharoyan S, Sengle G, Antonyan A, Bruckner-Tuderman L, Dengjel J, Reinhardt DP, Nystr枚m A. (2024). Dipeptidyl peptidase-4-mediated fibronectin processing evokes a pro-fibrotic extracellular matrix. J Invest Dermatol in press.
Zimmermann EA, DeVet T, Cilla M, Albiol L, Kavaseri K, Andrea C, C. J, Tiedemann K, Panahifar A, Chromik R, Komarova SV, Reinhardt DP, Zaslansky P, Willie BM. (2024). Tissue material properties, whole-bone morphology and mechanical behavior in the Fbn1 C1041G/+ mouse model of Marfan Syndrome. Matrix Biol Plus 23: 100155.
Lau C, Muthu ML, Siddiqui IF, Li L, Reinhardt DP. (2023). High-fat diet has a protective sex-dependent effect on aortic aneurysm severity in a Marfan syndrome mouse model. Can J Cardiol 39: 1553-1567.
Alonso F, Yuechao D, Li L, Jahjah T, Dupuy J-W, Fremaux I, #Reinhardt DP, #Genot EM. #Co-corresponding authors聽(2023). Fibrillin-1 regulates endothelial sprouting during angiogenesis. Proc. Natl. Acad. Sci. USA 120: e2221742120.
de Souza RB, Lemes RB, Foresto-Neto O, Cassiano LL, Reinhardt DP, Meek KM, Koh IHJ, Lewis PN, Pereira LV (2023). Extracellular matrix and vascular dynamics in the kidney of a murine model for Marfan syndrome. PLoS One 18: e0285418.
Alonso F, Li L, Fr茅maux I, Reinhardt DP, G茅not E. (2022). Fibrillin-1 regulates arteriole integrity in the retina. Biomolecules 12: 1330.
*Muthu ML, *Tiedemann K, Fradette J, #Komarova SV, #Reinhardt DP, *Co-first authors contributed equally to this work, #Co-corresponding authors. (2022). Fibrillin-1 regulates white adipose tissue development, homeostasis, and function. Matrix Biol 110: 106-128.
Zhang R-M, Tiedemann K, Dinesh NEH, Muthu ML, Komarova S, Ramkhelawon B, Reinhardt DP. (2022). Fibrillin-1 regulated miR-122 has a critical role in thoracic aortic aneurysm formation. Cell Mol Life Sci 79: 314.
*Tiedemann K, *Muthu ML, #Reinhardt DP, #Komarova SV, *Co-first authors contributed equally to this work, #Co-corresponding authors. (2022). Male Marfan mice are predisposed to high fat diet induced obesity, diabetes, and fatty liver. Am J Physiol Cell Physiol 323: C354-C366.
Dinesh NEH, Campeau PM, Reinhardt DP. (2022). Fibronectin isoforms in skeletal development and associated disorders. Am J Physiol Cell Physiol 323: C536-C549.
Dinesh NEH, Campeau PM, Reinhardt DP. (2022). Pathomechanisms in corner fracture type spondylometaphyseal dysplasia. MRM Insights.
Mead TJ, Martin DR, Wang LW, Cain, S. A., Gulec C, Cahill E, Mauch J, Reinhardt DP, Lo CW, Baldock C, Apte SS. (2022). Proteolysis of fibrillin-2 microfibrils is essential for normal skeletal development. eLife 11: e71142.
Boizot J, Minville-Walz M, Reinhardt DP, Bouschbacher M, Sommer P, Sigaudo-Roussel D, Debret R. (2022). FBN2 silencing recapitulates hypoxic conditions and induces elastic fiber impairment in human dermal fibroblasts. Int J Mol Sci 23: 1824.
Camas茫o D, Li L, Drouin B, Lau C, Reinhardt DP, Mantovani D. (2022). Physiologically relevant platform for an advanced in vitro model of the vascular wall: focus on in situ fabrication and mechanical maturation. In Vitro Models: 179-195.
Balic Z, Misra S, Willard B, Reinhardt DP, Apte SS, Hubmacher D. (2021). Alternative splicing of the metalloprotease ADAMTS17 spacer regulates secretion and modulates autoproteolytic activity. FASEB J 35: e21310.
C么t茅 E, Zhang R, Kaiser N, Reinhardt DP, Martin C. (2021). Annuloaortic ectasia in a dog: long-term follow-up and immunofluorescent study. Vet Q 41: 280-291.
de Souza RB, Gyuricza IG, Cassiano LL, Farinha-Arcieri LE, Alvim Liberatore AM, Schuindt do Carmo S, Caldeira W, Cruz MV, Ribeiro AF, Tedesco RC, Reinhardt DP, Smith R, Jun Koh IH, Pereira LV. (2021). The mg螖lpn mouse model for Marfan syndrome recapitulates the ocular phenotypes of the disease. Exp Eye Res 204: 108461.
de Souza RB, Kawahara EI, Farinha-Arcieri LE, Gerdes Gyuricza I, Neofiti-Papi B, Miranda-Rodrigues M, Cruz Grecco Teixeira M, Ribeiro Fernandes G, Barbarosa Lemes R, Reinhardt DP, Gouveia CH, Pereira LV. (2021). Hyperkyphosis is not dependent on bone mass and quality in the mouse model of Marfan syndrome. Bone 152: 116073.
Shi Y, Jones W, Beatty W, Tan Q, Mecham R, Kumra H, Reinhardt DP, Gibson MA, Reilly MA, Rodriguez J, Bassnett S. (2021). Latent-transforming growth factor beta-binding protein-2 (LTBP-2) is required for longevity but not for development of zonular fibers. Matrix Biol 95: 15-31.
Sun B, Tomita B, Salinger A, Tilvawala RR, Li L, Hakami H, Liu T, Tsoyi K, Rosas IO, Reinhardt DP, Thompson PR, Ho I-C. (2021). PAD2-mediated citrullination of fibulin-5 promotes elastogenesis. Matrix Biol 102: 70-84.
Zhang RM, Zeyer KA, Odenthal N, Zhang Y, Reinhardt DP. (2021). The fibrillin-1 RGD motif post-transcriptionally regulates ERK1/2 signaling and fibroblast proliferation via miR-1208. FASEB J 35: e21598.
Boel A, Burger J, Vanhomwegen M, Beyens A, Renard M, Barnhoorn S, Casteleyn C, Reinhardt DP, Descamps B, Vanhove C, van der Pluijm I, Coucke P, Willaert A, Essers J, Callewaert B. (2020). Slc2a10 knock-out mice deficient in ascorbic acid synthesis recapitulate aspects of arterial tortuosity syndrome and display mitochondrial respiration defects. Hum Mol Genet 29: 1476-1488.
Muthu ML, Reinhardt DP. (2020). Fibrillin-1 and fibrillin-1-derived asprosin in adipose tissue function and metabolic disorders. J Cell Commun Signal 14: 159-173.
Nelea V, Reinhardt DP. (2020). Biophysical techniques to analyze elastic tissue extracellular matrix proteins. Methods Mol Biol 2043: 213-235.
Zhang R, Kumra H, Reinhardt DP. (2020). Quantification of extracellular matrix fiber systems related to ADAMTS proteins. Methods Mol Biol 2043: 237-250.
*Kumra H, *Nelea V, Hakami H, Pagliuzza A, Djokic J, Xu J, Yanagisawa H, Reinhardt DP, *Co-first authors contributed equally to this work. (2019). Fibulin-4 exerts a dual role in LTBP-4L mediated matrix assembly and function. Proc Natl Acad Sci U S A 116: 20428-20437.
*Zeyer KA, *Zhang RM, Kumra H, Hassan A, Reinhardt DP, *Co-first authors contributed equally to this work. (2019). The fibrillin-1 RGD integrin binding site regulates gene expression and cell function through microRNAs. J Mol Biol 431: 401-421.
Burger J, van Vliet N, van Heijningen P, Kumra H, Kremers G-J, Alves M, van Cappellen G, Yanagisawa H, Reinhardt DP, Kanaar R, van der Pluijm I, Essers J. (2019). Fibulin-4 deficiency differentially affects cytoskeleton structure and dynamics as well as TGF尾 signaling. Cell Signal 58: 65-78.
Costantini A, Valta H, Baratang NV, Yap P, Bertola DR, Yamamoto GL, Kim CA, Chen J, Wierenga KJ, Fanning EA, Escobar L, McWalter K, McLaughlin H, Willaert R, Begtrup A, Alm JJ, Reinhardt DP, M盲kitie O, Campeau PM. (2019). Novel fibronectin mutations expand the phenotype of the FN1-subtype of spondylometaphyseal dysplasia with "corner fractures". Bone 121: 163-171.
Dinesh NEH, Reinhardt DP. (2019). Inflammation in thoracic aortic aneurysms. Herz 44: 138-146.
Kumra H, Dinesh NEH, Reinhardt DP. (2019). Lessons from tracheal tube development for understanding congenital tracheal malformations. Eur Respir J 53: 1900127.
Wang LW, Kutz WE, Mead TJ, Beene LC, Singh S, Jenkins MW, Reinhardt DP, Apte SS. (2019). Adamts10 inactivation in mice leads to persistence of ocular microfibrils subsequent to reduced fibrillin-2 cleavage. Matrix Biol 77: 117-128.
Yin X, Wanga S, Fellows AL, Barallobre-Barreiro J, Lu R, Davaapil H, Franken R, Fava M, Baig F, Skroblin P, Xing Q, Koolbergen DR, Groenink M, Zwinderman K, Balm R, de Vries CJ, Mulder BJ, Viner R, Jahangiri M, Reinhardt DP, Sinha S, de Waard V, Mayr M. (2019). Glycoproteomic analysis of the aortic extracellular matrix in Marfan patients. Arterioscler Thromb Vasc Biol 39: 1859-1873.
Camas茫o D, Pezzoli D, Loy C, Kumra H, Levesque L, Reinhardt DP, Candiani G, Mantovani D. (2018). Increasing cell seeding density improves elastin expression and mechanical properties in collagen gel-based scaffolds cellularized with smooth muscle cells. Biotechnol J 1700768.
Kumra H, Reinhardt DP. (2018). Fibrillins. Methods Cell Biol 143: 223-246.
Kumra H, Sabatier L, Hassan A, Sakai T, Mosher DF, Brinckmann J, Reinhardt DP. (2018). Roles of fibronectin isoforms in neonatal vascular development and matrix integrity. PLoS Biol 16: e2004812.
Lee K-WL, Gade PS, Dong L, Zhang Z, Mubin Aral A, Jin G, Ding X, Stowell CET, Umer Nisar M, Kim K, Reinhardt DP, Solari MG, Gorantla VS, Robertson AM, Wang Y. (2018). A biodegradable synthetic graft for small arteries matches the performance of autologous vein in rat carotid arteries. Biomaterials 181: 67-80.
Pezzoli D, Di Paolo J, Kumra H, Fois G, Candiani G, **Reinhardt DP, **Mantovani D, **Co-corresponding authors contributed equally to this work. (2018). Fibronectin promotes elastin deposition, elasticity and mechanical strength in cellularised collagen-based scaffolds. Biomaterials 180: 130-142.
Regalado ES, Mellor-Crummey L, De Backer J, Braverman AC, Ades L, Benedict S, Bradley TJ, Brickner ME, Chatfield KC, Child A, Feist C, Holmes KW, Iannucci G, Lorenz B, Mark P, Morisaki T, Morisaki H, Morris SA, Mitchell AL, Ostergaard JR, Richer J, Sallee D, Shalhub S, Tekin M, Montalcino Aortic Consortium**, Estrera A, Musolino P, Yetman A, Pyeritz R, Milewicz DM, **Reinhardt DP is member of this consortium. (2018). Clinical history and management recommendations of the smooth muscle dysfunction syndrome due to ACTA2 arginine 179 alterations. Genet Med 20: 1206-1215.
Gilpin SE, Li Q, Evangelista-Leite D, Ren X, Reinhardt DP, Frey BL, Ott HC. (2017). Fibrillin-2 and Tenascin-C bridge the age gap in lung epithelial regeneration. Biomaterials 140: 212-219.
Hubmacher D, Schneider M, Berardinelli SJ, Takeuchi H, Willard B, Reinhardt DP, Haltiwanger RS, Apte SS. (2017). Unusual life cycle and impact on microfibril assembly of ADAMTS17, a secreted metalloprotease mutated in genetic eye disease. Sci Rep 7: 41871.
Lee CS, Fu H, Baratang N, Rousseau J, Kumra H, Sutton VR, Niceta M, Ciolfi A, Yamamoto G, Bertola D, Marcelis CL, Lugtenberg D, Bartuli A, Kim C, Hoover-Fong J, Sobreira N, Pauli R, Bacino C, Krakow D, Parboosingh J, Yap P, Kariminejad A, McDonald MT, Aracena MI, Lausch E, Unger S, Superti-Furga A, Lu JT, Baylor-Hopkins Center for Mendelian Genomics, Cohn DH, Tartaglia M, Lee BH, **Reinhardt DP, **Campeau PM, **Co-corresponding authors contributed equally to this work. (2017). Mutations in fibronectin cause a subtype of spondylometaphyseal dysplasia with 鈥渃orner fractures鈥�. Am J Hum Genet 101: 815-823.
Zeyer KA, Reinhardt DP. (2017). Fibrillin-1 in connective tissues. In: Marfan Syndrome. Eds. Marfan Hilfe e. V. Springer, Berlin (Germany). Chp. 13, 64-66.
Jondeau G, Ropers J, Regalado E, Braverman A, Evangelista A, Teixedo G, De Backer J, Muino-Mosquera L, Naudion S, Zordan C, Morisaki H, Morisaki Y, von Kodolitsch Y, Dupuis-Girod S, Morris SA, Jeremy R, Odent S, Ades LC, Bakshi M, Holman K, LeMaire S, Milleron O, Langeois M, Spentchian M, Aubart M, Boileau C, Pyeritz R, Milewicz DM, Montalcino Aortic Consortium**, **Reinhardt DP is member of this consortium. (2016). International Registry of Patients Carrying TGFBR1 or TGFBR2 Mutations: Results of the Montalcino Aortic Consortium. Circ Cardiovasc Genet 9: 548-558.
Kahle B, Schmidtke C, Hunzelmann N, Bartels C, Sievers HH, Reinhardt DP, Brinckmann J. (2016). The extracellular matrix signature in vein graft disease. Can J Cardiol 32: 1008.e11-7.
Kumra H, Reinhardt DP. (2016). Fibronectin-targeted drug delivery in cancer. Adv Drug Deliv Rev 97: 101-110.
Li CD, Hancock MA, Seghal P, Zhou S, Reinhardt DP, Philip A. (2016). Soluble CD109 binds TGF-脽 and antagonizes TGF-脽 signaling and responses. Biochem J 473: 537-547.
Loy C, Meghezi S, L茅vesque L, Pezzoli D, Kumra H, Reinhardt DP, Kizhakkedathu JN, Mantovani D. (2016). A planar model of the vessel wall from cellularized collagen scaffolds: focus on cell鈥搈atrix interactions in mono-, bi- and tri-culture models. Biomater Sci 5: 153-162.
Schaefer L, Reinhardt DP. (2016). Extracellular matrix: Therapeutic tools and targets in cancer treatment. Adv Drug Deliv Rev 97: 1-3.
Guo DC, Gong L, Regalado ES, Santos-Cortez RL, Zhao R, Cai B, Veeraraghavan S, Prakash SK, Johnson RJ, Muilenburg A, Willing M, Jondeau G, Boileau C, Pannu H, Moran R, Debacker J, GenTac Investigators National Heart Lung Blood Institute Go Exome Sequencing Project, Montalcino Aortic Consortium**, Bamshad MJ, Shendure J, Nickerson DA, Leal SM, Raman CS, Swindell EC, Milewicz DM, **Reinhardt DP is member of this consortium. (2015). MAT2A mutations predispose individuals to thoracic aortic aneurysms. Am J Hum Genet 96: 170-177.
Hinderer S, Shena N, Ringuette LJ, Hansmann J, Reinhardt DP, Brucker SY, Davis EC, Schenke-Layland K. (2015). In vitro elastogenesis - Instructing human vascular smooth muscle cells to generate an elastic fiber-containing extracellular matrix scaffold. Biomed Mater 10: 034102.
Hubmacher D, Wang LW, Mecham RP, Reinhardt DP, Apte SS. (2015). Adamtsl2 deletion results in bronchial fibrillin microfibril accumulation and bronchial epithelial dysplasia: A novel mouse model providing insights on geleophysic dysplasia. Dis Model Mech 8: 487-499.
Kaur J, Reinhardt DP. (2015). Extracellular Matrix Molecules. In: Stem Cell Biology and Tissue Engineering in Dental Science. Eds. Vishwakarma A, Sharpe PT, Shi S, Wang X, Ramalingam M. Elsevier, New York. Chp. 3, 25-43.
Regalado ES, Guo DC, Prakash S, Bensend TA, Flynn K, Estrera A, Safi H, Liang D, Hyland J, Child A, Arno G, Boileau C, Jondeau G, Braverman A, Moran R, Morisaki T, Morisaki H, Montalcino Aortic Consortium**, Pyeritz R, Coselli J, LeMaire S, Milewicz DM, **Reinhardt DP is member of this consortium. (2015). Aortic disease presentation and outcome associated with ACTA2 mutations. Circ Cardiovasc Genet 8: 457-464.
Zeyer KA, Reinhardt DP. (2015). Fibrillin-containing microfibrils are key signal relay stations for cell function. J Cell Commun Signal 9: 309-325.
Zeyer KA, Reinhardt DP. (2015). Engineered mutations in fibrillin-1 leading to Marfan syndrome act at the protein and cellular levels. Mutat Res Rev 765: 7-18.
Hubmacher D, Bergeron E, Fagotto-Kaufmann C, Sakai LY, Reinhardt DP. (2014). Early fibrillin-1 assembly monitored through a modifiable recombinant cell approach. Biomacromolecules 15: 1456-1468.
Hubmacher D, Reinhardt DP, Plesec T, Schenke-Layland K, Apte SS. (2014). Human eye development is characterized by coordinated expression of fibrillin isoforms. Invest Ophthalmol Vis Sci 55: 7934-7944.
Marek I, Volkert G, Hilgers KF, Bieritz B, Rascher W, Reinhardt DP, Hartner A. (2014). Fibrillin-1 and alpha8 integrin are co-expressed in the glomerulus and interact to convey firm adhesion of mesangial cells. Cell Adh Migr 8: 389-395.
Muiznieks LD, Cirulis JT, van der Horst A, Reinhardt DP, Wuite GJL, Pom猫s R, Keeley FW. (2014). Modulated growth, stability and interactions of liquid-like coacervate assemblies of elastin. Matrix Biol 36: 39-50.
Murphy EC, M枚rgelin M, Reinhardt DP, Bj枚rck L, Frick IM. (2014). Finegoldia magna uses its cell surface and extracellular proteins, FAF and SufA, to colonise and pass into deeper fractions of human skin. Mol Microbiol 94: 403-417.
Reinhardt DP. (2014). Microfibril-associated disorders: fibrillinopathies. J Glaucoma 23: S34-S35.
Sabatier L, Djokic J, Hubmacher D, Dzafik D, Nelea V, Reinhardt DP. (2014). Heparin/heparan sulfate controls fibrillin-1, -2 and -3 self-interactions in microfibril assembly. FEBS Lett 588: 2890-2897.
Beene LC, Wang LW, Hubmacher D, Keene DR, Reinhardt DP, Annis DS, Mosher DF, Mecham RP, Traboulsi EI, Apte SS. (2013). Non-selective assembly of fibrillin-1 and fibrillin-2 in the rodent ocular zonule and in cultured cells: Implications for Marfan syndrome. Invest Ophthalmol Vis Sci 54: 8337-8344.
Djokic J, Fagotto-Kaufmann C, Bartels R, Nelea V, Reinhardt DP. (2013). Fibulin-3, -4, and -5 are highly susceptible to proteolysis, interact with cells and heparin, and form multimers. J Biol Chem 288: 22821-22835.
Hummitzsch K, Irving-Rodgers HF, Hatzirodos N, Bonner W, Sabatier L, Reinhardt DP, Sado Y, Ninomiya Y, Wilhelm D, Rodgers RJ. (2013). A new model of development of the mammalian ovary and follicles. PLoS One 8: e55578.
Sabatier L, Djokic J, Fagotto-Kaufmann C, Chen M, Annis DS, Mosher DF, Reinhardt DP. (2013). Complex contributions of fibronectin to initiation and maturation of microfibrils. Biochem J 456: 283-295.
Tiedemann K, Boraschi-Diaz I, Rajakumar I, Kaur J, Roughley P, **Reinhardt DP, **Komarova SV, **Co-senior authors contributed equally to this work. (2013). Fibrillin-1 directly regulates osteoclast formation and function by a dual mechanism. J Cell Sci 126: 4187-4194.
Votteler M, Carvaja Berrio DA, Horke A, Sabatier L, Reinhardt DP, Nsair A, Aikawa E, Schenke-Layland K. (2013). Elastogenesis at the onset of human cardiac valve development. Development 140: 2345-2353.
Bader HL, Wang LW, Ho J, Tran T, Holden P, Fitzgerald J, Atit RP, Reinhardt DP, Apte SS. (2012). A disintegrin-like and metalloprotease domain containing thrombospondin type 1 motif-like 5 (ADAMTSL5) is a novel fibrillin-1-, fibrillin-2-, and heparin-binding member of the ADAMTS superfamily containing a netrin-like module. Matrix Biol 31: 398-411.
Kaur J, Reinhardt DP. (2012). Immobilized metal affinity chromatography co-purifies TGF-beta1 with histidine-tagged recombinant extracellular proteins. PLoS One 7: e48629.
Lammers G, Roth G, Heck M, Zengerle R, Tjabringa GS, Versteeg EM, Hafmans T, Wismans R, Reinhardt DP, Verwiel ET, Verwiel E, Zeeuwen PL, Schalkwijk J, Brock R, Daamen WF, van Kuppevelt TH. (2012). Construction of a microstructured collagen membrane mimicking the papillary dermis architecture and guiding keratinocyte morphology and gene expression. Macromol Biosci 12: 675-691.
Piha-Gossack A, Sossin W, Reinhardt DP. (2012). The evolution of extracellular fibrillins and their functional domains. PLoS One 7: e33560.
Hatzirodos N, Bayne RA, Irving-Rodgers HF, Hummitzsch K, Sabatier L, Lee S, Bonner W, Gibson MA, Rainey WE, Carr BR, Mason HD, Reinhardt DP, Anderson RA, Rodgers R. (2011). Linkage of regulators of TGF-beta activity in the fetal ovary to polycystic ovary syndrome. FASEB J 25: 2256-2265.
Hubmacher D, Reinhardt DP. (2011). Microfibrils and fibrillin. In: The Extracellular Matrix: an Overview. Eds. Mecham RP. Springer, Berlin. Chp. 7, 233-265.
Hubmacher D, Sabatier L, Annis DS, Mosher DF, Reinhardt DP. (2011). Homocysteine modifies structural and functional properties of fibronectin and interferes with the fibronectin-fibrillin-1 interaction. Biochemistry 50: 5322-5332.
Kirschner R, Hubmacher D, Iyengar G, Kaur J, Fagotto-Kaufmann C, Bromme D, Bartels R, Reinhardt DP. (2011). Classical and neonatal Marfan syndrome mutations in fibrillin-1 cause differential protease susceptibilities and protein function. J Biol Chem 286: 32810-32823.
Sabatier L, Miosge N, Hubmacher D, Lin G, Davis EC, Reinhardt DP. (2011). Fibrillin-3 expression in human development. Matrix Biol 30: 43-52.
Brinckmann J, Hunzelmann N, Kahle B, Rohwedel J, Kramer J, Hubmacher D, Reinhardt DP. (2010). Enhanced fibrillin-2 expression is a general feature of wound healing and sclerosis: Potential alteration of cell attachment and storage of TGF-beta. Lab Invest 90: 739-752.
Hubmacher D, Cirulis JT, Miao M, Keeley FW, Reinhardt DP. (2010). Functional consequences of homocysteinylation of the elastic fiber proteins fibrillin-1 and tropoelastin. J Biol Chem 285: 1188-1198.
Schenke-Layland K, Xie J, Magnusson M, Angelis E, Li X, Wu K, Reinhardt DP, MacLellan WR, Hamm-Alvarez SF. (2010). Lymphocytic infiltration leads to degradation of lacrimal gland extracellular matrix structures in NOD mice exhibiting a Sj枚gren's syndrome-like exocrinopathy. Exp Eye Res 90: 223-237.
Hubmacher D, Reinhardt DP. (2009). One more piece in the fibrillin puzzle. Structure 7: 635-636.
Koenders MMJF, Yang L, Wismans RG, van der Werf KO, Reinhardt DP, Daamen W, Bennink ML, Dijkstra PJ, van Kuppevelt TH, Feijen J. (2009). Microscale mechanical properties of single elastic fibers: The role of fibrillin-microfibrils. Biomaterials 30: 2425-2432.
Sabatier L, Chen D, Fagotto-Kaufmann C, Hubmacher D, McKee MD, Annis DS, Mosher DF, Reinhardt DP. (2009). Fibrillin assembly requires fibronectin. Mol Biol Cell 20: 846-858.
Sabatier L, Chen D, Fagotto-Kaufmann C, Hubmacher D, McKee MD, Annis DS, Mosher DF, Reinhardt DP. (2009). Fibrillin assembly requires fibronectin - Incytes Highlight. Mol Biol Cell 20: 1119-1119.
Cirulis JT, Bellingham CM, Davis EC, Hubmacher D, Reinhardt DP, Mecham RP, Keeley FW. (2008). Fibrillins, fibulins and MAGP modulate the kinetics and morphology of in vitro self-assembly of a recombinant elastin-like polypeptide. Biochemistry 47: 12601-12613.
Daamen WF, Nillesen STM, Wismans R, Reinhardt DP, Hafmans T, Veerkamp JH, van Kuppevelt T. (2008). A biomaterial composed of collagen and solubilised elastin enhances angiogenesis and elastic fibre formation without calcification. Tissue Eng 14: 349-360.
Fricke M, Langer C, Brunner E, Sakai LY, F眉zesi L, Reinhardt DP, Quondamatteo F. (2008). Fibrillin-1 in incisional hernias. An immunohistochemical study in scar and non-scar regions of human skin and muscle fasciae. J Anat 212: 674-685.
Hubmacher D, El-Hallous EI, Nelea V, Kaartinen MT, Lee ER, Reinhardt DP. (2008). Biogenesis of extracellular microfibrils-Multimerization of the fibrillin-1 C-terminus into bead-like structures enables self-assembly. Proc Natl Acad Sci U S A 105: 6548-6553.
Kloepper JE, Tiede S, Brinckmann J, Reinhardt DP, F盲ssler R, Paus R. (2008). Immunophenotyping of the human bulge region: The quest to define useful in situ markers for human epithelial hair follicle stem cells and their niche. Exp Dermatol 17: 592-609.
Wilkinson GA, Schittny JC, Reinhardt DP, Klein R. (2008). Role for ephrinB2 in postnatal lung alveolar development and elastic matrix integrity. Dev Dyn 237: 2220-2234.
El-Hallous EI, Sasaki T, Hubmacher D, Getie M, Tiedemann K, Brinckmann J, B盲tge B, Davis EC, Reinhardt DP. (2007). Fibrillin-1 interactions with fibulins depend on the first hybrid domain and provide an adapter function to tropoelastin. J Biol Chem 282: 8935-8946.
Gnainsky Y, Kushnirsky Z, Bilu G, Hagai Y, Genina O, Volpin H, Bruck R, Spira G, Nagler A, Kawada N, Yoshizato K, Reinhardt DP, Libermann TA, Pines M. (2007). Gene expression during chemically induced liver fibrosis - effect of halofuginone on TGF-beta signaling. Cell Tissue Res 328: 153-166.
Schaefer L, Tsalastra W, Babelova A, Baliova M, Minnerup J, Sorokin L, Gr枚ne HJ, Reinhardt DP, Pfeilschifter J, Iozzo RV, Schaefer RM. (2007). Decorin-mediated regulation of fibrillin-1 in the kidney involves the IGF-I receptor and mTOR. Am J Pathol 170: 301-315.
Drumea-Mirancea M, Wessels JT, M眉ller CA, Essl M, Eble JA, Tolosa M, Koch M, Reinhardt DP, Sixt M, Sorokin L, Stierhof YD, Schwarz H, Klein G. (2006). Characterization of a laminin-5 containing conduit system in the human thymus - a transport system for small molecules. J Cell Sci 119: 1396-1405.
Hartner A, Schaefer L, Porst M, Cordasic N, Gabriel A, Klanke B, Reinhardt DP, Hilgers KF. (2006). Role of fibrillin-1 in hypertensive and diabetic glomerular disease. American Journal of Physiology: Renal Physiology 290: F1329-F1336.
Hubmacher D, Tiedemann K, Reinhardt DP. (2006). Fibrillins: From biogenesis of microfibrils to signaling functions. Curr Top Dev Biol 75: 93-123.
Porst M, Daniel C, Plank C, Schocklmann HO, Reinhardt DP, Hartner A. (2006). Induction and coexpression of latent transforming growth factor beta-binding protein 1 (LTBP-1) and fibrillin-1 in experimental glomerulonephritis. Nephron Exp Nephrol 102: e99-e104.
Porst M, Plank C, Bieritz B, Konik E, Fees H, D枚tsch J, Hilgers KF, Reinhardt DP, Hartner A. (2006). Fibrillin-1 regulates mesangial cell attachment, spreading, migration and proliferation. Kidney Int 69: 450-456.
Reinhardt DP. (2006). Connective Tissue. In: Marfan-Syndrom - Ein Ratgeber f眉r Patienten, Angeh枚rige und Betreuende. Eds. Marfan Hilfe e. V. Steinkopff Verlag, Darmstadt (Germany). Chp. 6, 26-33.
Robinson P, Arteaga-Solis E, Baldock C, Collod-Beroud G, Booms P, De Paepe A, Dietz HC, Guo G, Handford PA, Judge DP, Kielty CM, Loeys B, Milewicz DM, Ney A, Ramirez F, Reinhardt DP, Tiedemann K, Whiteman P, Godfrey M. (2006). The molecular genetics of Marfan syndrome and related disorders. J Med Genet 43: 769-787.
Robinson PN, Reinhardt DP. (2006). Research Outlook. In: Marfan-Syndrom - Ein Ratgeber f眉r Patienten, Angeh枚rige und Betreuende. Eds. Marfan Hilfe e. V. Steinkopff Verlag, Darmstadt (Germany). Chp. 19, 143-148.
Wu J, Reinhardt DP, Batmunkh C, Lindenmaier W, Kretschmer-Kazemi Far R, Notbohm H, Hunzelmann N, Brinckmann J. (2006). Functional diversity of lysyl hydroxylase 2 in collagen synthesis of human dermal fibroblasts. Exp Cell Res 312: 3485-3494.
Booms P, Pregla R, Ney A, Barthel F, Reinhardt DP, Pletschacher A, Mundlos S, Robinson PN. (2005). RGD-containing fibrillin-1 fragments upregulate matrix metalloproteinase expression in cell culture: A potential factor in the pathogenesis of the Marfan syndrome. Hum Genet 116: 51-61.
Bouzeghrane F, Reinhardt DP, Reudelhuber T, Thibault G. (2005). Enhanced expression of fibrillin-1, a constituent of the myocardial extracellular matrix, in fibrosis. American Journal of Physiology: Heart and Circulatory Physiology 289: H982-H991.
Brinckmann J, Hunzelmann N, El-Hallous EI, Krieg T, Sakai LY, Krengel S, Reinhardt DP. (2005). Absence of autoantibodies against correctly folded recombinant fibrillin-1 protein in systemic scleroderma patients. Arthritis Res Ther 7: R1221-R1226.
Brinckmann J, Kim S, Wu J, Reinhardt DP, Batmunkh C, Metzen E, Notbohm H, Bank RA, Krieg T, Hunzelmann N. (2005). Interleukin 4 and prolonged hypoxia induce a higher gene expression of lysyl hydroxylase 2 and an altered cross-link pattern: Important pathogenetic steps in early and late stage of systemic scleroderma? Matrix Biol 24: 459-468.
Hubmacher D, Tiedemann K, Bartels R, Brinckmann J, Vollbrandt T, B盲tge B, Notbohm H, Reinhardt DP. (2005). Modification of the structure and function of fibrillin-1 by homocysteine suggests a potential pathogenetic mechanism in homocystinuria. J Biol Chem 280: 34946-34955.
Sixt M, Kanazawa N, Selg M, Samson T, Roos G, Reinhardt DP, Pabst R, Lutz MB, Sorokin L. (2005). The conduit system transports soluble antigens from the afferent lymph to resident dendritic cells in the T cell area of the lymph node. Immunity 22: 19-29.
Tiedemann K, Sasaki T, Gustafsson E, G枚hring W, B盲tge B, Notbohm H, Timpl R, Wedel T, Schl枚tzer-Schrehardt U, Reinhardt DP. (2005). Microfibrils at basement membrane zones interact with perlecan via fibrillin-1. J Biol Chem 280: 11404-11412.
Gaber Y, Tiedemann K, Reinhardt DP, Brinckmann J. (2004). Changes in crosslinks in the collagenous connective tissue of lipodermatosclerosis. Phlebologie 33: 8-11.
Hartner A, Eifert T, Haas CS, Tuysuz C, Hilgers KF, Reinhardt DP, Amann K. (2004). Characterization of the renal phenotype in a mouse model of Marfan Syndrome. Virchows Arch 445: 382-388.
Kassner A, Tiedemann K, Notbohm H, Ludwig T, M枚rgelin M, Reinhardt DP, Chu ML, Bruckner P, Gr盲ssel S. (2004). Molecular structure and interaction of recombinant human type XVI collagen. J Mol Biol 339: 835-853.
Lorena D, Darby IA, Reinhardt DP, Sapin V, Rosenbaum J, Desmouliere A. (2004). Fibrillin-1 expression in normal and fibrotic rat liver and in cultured hepatic fibroblastic cells: Modulation by mechanical stress and role in cell adhesion. Lab Invest 84: 203-212.
Schaefer L, Mihalik D, Babelova A, Krzyzankova M, Grone HJ, Iozzo RV, Young MF, Seidler DG, Lin G, Reinhardt DP, Schaefer RM. (2004). Regulation of fibrillin-1 by biglycan and decorin is important for tissue preservation in the kidney during pressure-induced injury. Am J Pathol 165: 383-396.
Tiedemann K, B盲tge B, Reinhardt DP. (2004). Assembly of microfibrils. In: Marfan Syndrome: A Primer for Clinicians and Scientists. Eds. Robinson PN, Godfrey M. Landes Bioscience, Georgetown, Texas, USA. Chp., 130-142.
Vollbrandt T, Tiedemann K, El-Hallous EI, Lin G, Brinckmann J, John H, B盲tge B, Notbohm H, Reinhardt DP. (2004). Consequences of cysteine mutations in calcium-binding epidermal growth factor modules of fibrillin-1. J Biol Chem 279: 32924-32931.
Charbonneau NL, Dzamba BJ, Ono RN, Keene DR, Corson GM, Reinhardt DP, Sakai LY. (2003). Fibrillins can co-assemble in fibrils, but fibrillin fibril composition displays cell-specific differences. J Biol Chem 278: 2740-2749.
Eisenbeiss C, Martinez A, Hagedorn-Greiwe M, Reinhardt DP, B盲tge B, Brinckmann J. (2003). Reduced skin thickness - a "new" minor symptom of the classical and hypermobile type of Ehlers-Danlos syndrome. Br J Dermatol 149: 850-852.
Isogai Z, Ono RN, Ushiro S, Keene DR, Chen Y, Mazzieri R, Charbonneau NL, Reinhardt DP, Rifkin DB, Sakai LY. (2003). Latent transforming growth factor beta-binding protein 1 interacts with fibrillin and is a microfibril-associated protein. J Biol Chem 278: 2750-2757.
Kassner A, Hansen U, Miosge N, Reinhardt DP, Aigner T, Bruckner-Tuderman L, Bruckner P, Gr盲ssel S. (2003). Discrete integration of collagen XVI into tissue-specific collagen fibrils or beaded microfibrils. Matrix Biol 22: 131-143.
Tiedemann K, B盲tge B, Reinhardt DP. (2003). Marfan-Syndrom: Eine genetische Erkrankung des mikrofibrill盲ren Systems. Bioforum 7-8: 456-457.
Isogai Z, Aspberg A, Keene DR, Ono RN, Reinhardt DP, Sakai LY. (2002). Versican interacts with fibrillin-1 and links extracellular microfibrils to other connective tissue networks. J Biol Chem 277: 4565-4572.
Lin G, Tiedemann K, Vollbrandt T, Peters H, B盲tge B, Brinckmann J, Reinhardt DP. (2002). Homo- and heterotypic fibrillin-1 and -2 interactions constitute the basis for the assembly of microfibrils. J Biol Chem 277: 50795-50804.
Piecha D, Wiberg C, M枚rgelin M, Reinhardt DP, Deak F, Maurer P, Paulsson M. (2002). Matrilin-2 interacts with itself and with other extracellular matrix proteins. Biochem J 367: 715-721.
Quondamatteo F, Reinhardt DP, Charbonneau NL, Pophal G, Sakai LY, Herken R. (2002). Fibrillin-1 and fibrillin-2 in human embryonic and early fetal development. Matrix Biol 21: 637-646.
Areida SK, Reinhardt DP, M眉ller PK, Fietzek PP, K枚witz J, Marinkovich MP, Notbohm H. (2001). Properties of the collagen type XVII ectodomain. Evidence for N- to C-terminal triple helix folding. J Biol Chem 276: 1594-1601.
Jensen SA, Reinhardt DP, Gibson MA, Weiss AS. (2001). MAGP-1, Protein interaction studies with tropoelastin and fibrillin-1. J Biol Chem 276: 39661-39666.
Tiedemann K, B盲tge B, M眉ller PK, Reinhardt DP. (2001). Interactions of fibrillin-1 with heparin/heparan sulfate: Implications for microfibrillar assembly. J Biol Chem 276: 36035-36042.
Winkler J, Wirbelauer C, L眉nsdorf H, Reinhardt DP, Laqua H. (2001). Immunohistochemical and charge-specific localization of anionic components of aggregates on the central anterior lens capsules from individuals with Pseudoexfoliation syndrome. Graefes Arch Clin Exp Ophthalmol 239: 952-960.
Handford PA, Downing AK, Reinhardt DP, Sakai LY. (2000). Fibrillin: from domain structure to supramolecular assembly. Matrix Biol 19: 457-470.
Reinhardt DP, Gambee JE, Ono RN, B盲chinger HP, Sakai LY. (2000). Initial steps in assembly of microfibrils. Formation of disulfide-cross-linked multimers containing fibrillin-1. J Biol Chem 275: 2205-2210.
Reinhardt DP, Ono RN, Notbohm H, M眉ller PK, B盲chinger HP, Sakai LY. (2000). Mutations in calcium-binding epidermal growth factor modules render fibrillin-1 susceptible to proteolysis: A potential disease-causing mechanism in Marfan syndrome. J Biol Chem 275: 12339-12345.
Wirbelauer C, Reinhardt DP, L眉nsdorf H, Weber E, Laqua H, Winkler J. (2000). Charakterisierung der extrazellul盲ren Matrix beim Pseudoexfoliationssyndrom. Focus MUL 17: 82-87.
L枚nnqvist L, Reinhardt DP, Sakai LY, Peltonen L. (1998). Evidence for furin-type activity-mediated C-terminal processing of profibrillin-1 and interference in the processing by certain mutations. Hum Mol Genet 7: 2039-2044.
Keene DR, Jordan CD, Reinhardt DP, Ridgway CC, Ono RN, Corson GM, Fairhurst M, Sussman MD, Memoli VA, Sakai LY. (1997). Fibrillin-1 in human cartilage: developmental expression and formation of special banded fibers. J Histochem Cytochem 45: 1069-1082.
Pereira L, Andrikopoulos K, Tian J, Lee SY, Keene DR, Ono RN, Reinhardt DP, Sakai LY, Jensen-Biery N, Bunton T, Dietz HC, Ramirez F. (1997). Targeting of fibrillin-1 recapitulates the vascular phenotype of Marfan syndrome in the mouse. Nat Genet 17: 218-222.
Reinhardt DP, Mechling DE, Boswell BA, Keene DR, Sakai LY, B盲chinger HP. (1997). Calcium determines the shape of fibrillin. J Biol Chem 272: 7368-7373.
Reinhardt DP, Ono RN, Sakai LY. (1997). Calcium stabilizes fibrillin-1 against proteolytic degradation. J Biol Chem 272: 1231-1236.
Pfaff M, Reinhardt DP, Sakai LY, Timpl R. (1996). Cell adhesion and integrin binding to recombinant human fibrillin-1. FEBS Lett 384: 247-250.
Reinhardt DP, Keene DR, Corson GM, P枚schl E, B盲chinger HP, Gambee JE, Sakai LY. (1996). Fibrillin 1: organization in microfibrils and structural properties. J Mol Biol 258: 104-116.
Reinhardt DP, Sasaki T, Dzamba BJ, Keene DR, Chu ML, G枚hring W, Timpl R, Sakai LY. (1996). Fibrillin-1 and fibulin-2 interact and are colocalized in some tissues. J Biol Chem 271: 19489-19496.
Mayer U, Zimmermann K, Mann K, Reinhardt DP, Timpl R, Nischt R. (1995). Binding properties and protease stability of recombinant human nidogen. Eur J Biochem 227: 681-686.
Reinhardt DP, Chalberg SC, Sakai LY. (1995). The structure and function of fibrillin. In: The molecular biology and pathology of elastic tissues. Eds. John Wiley and Sons, Chichester, England. Chp., 128-147.
Aumailley M, Battaglia C, Mayer U, Reinhardt DP, Nischt R, Timpl R, Fox JW. (1993). Nidogen mediates the formation of ternary complexes of basement membrane components. Kidney Int 43: 7-12.
Reinhardt DP, Mann K, Nischt R, Fox JW, Chu ML, Krieg T, Timpl R. (1993). Mapping of nidogen binding sites for collagen type IV, heparan sulfate proteoglycan, and zinc. J Biol Chem 268: 10881-10887.
K眉bler D, Reinhardt DP, Reed J, Pyerin W, Kinzel V. (1992). Atrial natriuretic peptide is phosphorylated by intact cells through cAMP-dependent ecto-protein kinase. Eur J Biochem 206: 179-186.
Fox JW, Mayer U, Nischt R, Aumailley M, Reinhardt DP, Wiedemann H, Mann K, Timpl R, Krieg T, Engel J, Chu ML. (1991). Recombinant nidogen consists of three globular domains and mediates binding of laminin to collagen type IV. EMBO J 10: 3137-3146.