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Sperm Cryopreservation

Protect your precious mouse lines against loss from unforeseen accident, disease or infertility due to aging.

• Requires only 2-3 males, 20-30 samples are stored
• Inexpensive and rapid
• Can use frozen sample for speed expansion or genome editing via CRISPR

Sperm and embryo cryorecovery

• Can recover samples from in house or external sources
• Excellent way to transfer out mouse lines or receiveÌý mouseÌý lines from other labs

CRISPR/Cas9 mediated genome editing

• Total gene KO in one generation with 50 - 100% efficiency
• Point mutations, add tags, or make small deletions (frequency 10-30% in one generation)
• Generate conditional KO alleles (no ES cells, no complicated targeting constructs)
• Targeted knock-ins (GFP, Cre etc)

Chimeric mouse production

• Recover GM mice from modified ES cells (eg.condtionals from consortiums)
• Test pluipotency of iPS cells
• Generate genetically mosaic animals
• Tetraploid complementation

Transgenic mouse production via pronuclear microinjection

• For large gene insertions, choose the traditional method of transgenic mouse production Ìý ÌýÌý
• Random integration of multiple copies of your transgene resulting in overexpression.