Using a new technique, a team of 不良研究所 researchers has found tiny and previously undetectable 鈥榟ot spots鈥� of extremely high stiffness inside aggressive and invasive breast cancer tumours. Their findings suggest, for the first time, that only very tiny regions of a tumor need to stiffen for metastasis to take place. Though still in its infancy, the researchers believe that their technique may prove useful in detecting and mapping the progression of aggressive cancers.
鈥淲e are now able to see these features because our approach allows us to take measurements within living, intact, 3D tissues,鈥� says Chris Moraes, from 不良研究所鈥檚 Department of Chemical Engineering, a Canada Research Chair and senior author on a recent research paper in Nature Communications. 鈥淲hen tissue samples are disrupted in any way, as is normally required with standard techniques, signs of these 鈥榟ot spots鈥� are eliminated.鈥�
鈥淪mart鈥� hydrogels provide information about cancer progression
The researchers built tiny hydrogel sensors that can expand on demand, much like inflating balloons the size of individual cells, and placed them inside 3D cultures and mouse models of breast cancer. When triggered, the expansion of the hydrogel can be used to measure very local stiffness inside the tumour.
This unusual technique, developed through a collaboration between 不良研究所鈥檚 Department of Chemical Engineering and the Rosalind and Morris Goodman Cancer Research Centre at 不良研究所, allows the researchers to sense, from the perspective of a cancer cell, what is going on in their surrounding environment.
What cells sense drives their behaviour
鈥淗uman cells are not static. They grab and pull on the tissue around them, checking out how rigid or soft their surroundings are. What cells feel around them typically drives their behaviour: immune cells can activate, stem cells can become specialized, and cancer cells can become dangerously aggressive,鈥� explains Moraes. 鈥淏reast cancer cells usually feel surroundings that are quite soft. However, we found that cancer cells inside aggressive tumours experienced much harder surroundings than previously expected, as hard as really old and dried up gummy bears.鈥�
The researchers believe that their findings suggest new ways in which cell mechanics, even at the early stages of breast cancer, might affect disease progression.
鈥淒eveloping methods to analyze the mechanical profiles in 3D tissues may better predict patient risk and outcome,鈥� says Stephanie Mok, the first author on the paper and a PhD candidate in the Department of Chemical Engineering. 鈥淲hether these 鈥榟ot spots鈥� of stiffness are really causing cancer progression rather than simply being correlated with it remains an open, but critically important question to resolve.鈥�