Cheese producers were cheesed off.聽 People were just not eating enough veal.聽 Slaughterhouses were running short of calf stomachs and the cheese industry was feeling the pinch.聽 There was not enough rennet to meet the demands of turophiles (that's "cheese lovers" from the Greek 鈥渢uro鈥� for cheese) around the world.
Rennet, you see, is critical to the cheese making process.聽 At least if you want to indulge in something that is a little more exciting than cottage cheese.聽 Traditionally, it has been made by washing, drying, macerating and brining the lining taken from the fourth stomach of calves.聽 This leads to a product that is a mixture of two enzymes, chymosin and bovine pepsin, both of which can coagulate milk by cleaving proteins in milk and causing them to precipitate. That precipitate is cheese!聽 Why does the stomach lining of mammals contain these enzymes?聽 Because they are needed for proper digestion!聽 If milk did not coagulate to some extent in the stomach, it would flow through the digestive tract too quickly and its proteins would not be sufficiently broken down into absorbable amino acids.
Chymosin is the ideal enzyme for catalyzing this process, but by the 1960s the shortage of rennet was becoming critical.聽 The stomachs of older animals were pressed into service, but the resulting rennet was not really suitable.聽 That鈥檚 when researchers discovered that certain fungi,聽Mucor miehei聽being a prime example, were capable of producing enzymes that would cleave proteins much like chymosin.聽 This meant that cheese could be produced without using any animal rennet at all.聽 Not only did this address the rennet shortage, it also made possible the production of cheese that met the needs of vegetarians.聽 Such cheese was also kosher because there was no mixing of milk and meat.聽 But purists claim that the taste was not the same.聽 They may well be right.聽 The fungal enzymes have greater proteolytic (protein breaking) activity than chymosin and can give rise to 鈥渙ff鈥� flavors.
Then genetic engineering entered the picture and essentially solved the chymosin problem.聽 The bit of DNA, the gene, that gives the instructions for the formation of chymosin was isolated from calf cells and was copied, or "cloned."聽 It was then successfully inserted into the genetic machinery of certain bacteria (E. coli), yeasts (Kluyveromyces lactis) or fungi (Aspergillus niger) which dutifully churned out pure chymosin.聽 Approved in 1990 by the Food and Drug Administration in the U.S., chymosin became the first product of genetic engineering in our food supply.聽 It is 100% identical to that found in calf stomach, but because it does not come from animals it is acceptable to consumers who do not want meat products in their cheese.聽
Extraordinary precautions were taken before chymosin, made by recombinant DNA technology, was marketed.聽 Regulators ensured that no toxins of any kind had been introduced, and that no live recombinant organisms were present.聽 Indeed, the product contained nothing but pure chymosin.聽 Cheese made with it is completely indistinguishable from that produced with animal rennet.聽 In any case, chymosin itself is degraded during cheese making and none is left in the finished product.聽 Today, in North America, over 80% of all cheese is made using chymosin produced by recombinant DNA technology.聽 Cheese makers no longer have to worry about a shortage of calf stomachs and turophiles can satisfy their critical tastebuds.聽 Thanks to biotechnology they can "say cheese" and smile.
