Animal and Request Information
We recommend you to provide 3 males, between 3-8 months old and proven breeders. This procedure is terminal. Sperm quality and success of sperm cryopreservation depend on many factors. We strongly suggest that you follow our protocol on how to prepare the males to optimize results.
Billing information
Please return the completed form and we will send you back a final quotation for the services. Once you agree with the quotation, we will pass all billing using a PO or FOAPAL number. If you provide a PO please send us a soft copy and the FOAPAL will be processed through MMP (不良研究所 Marketplace).
Description of Services
Sperm Cryopreservation, without Quality Control
- This service includes the sperm cryopreservation service with a quality control test to verify that the sperm is still alive and motile after thawing from liquid nitrogen. One straw is thawed and tested to verify that the sperm is still motile and alive. This does not give any guarantee about the sperm鈥檚 ability to fertilize oocytes. We strongly recommend the control by IVF before eliminating a mouse line from your colony.
Sperm Cryopreservation with Quality Control by IVF (recommended)
- This quality control tests the ability of the sperm to fertilize eggs and verifies our ability to recover a line and generate live animals.
- This service includes sperm cryopreservation, in vitro fertilization (IVF), culture of embryos from 2cell stage to blastocysts and determination of fertilization rate for future recovery.
- This service does not include the price of donor females for IVF since this will vary based on strain choice by the researcher. We will quote you the cost to purchase females for this QC test.
Sperm Cryopreservation with Quality Control to generate pups (P0-P3)
- This quality control test will test the ability of the sperm to fertilize eggs and generate live pups.
- This service includes sperm recovery and in vitro fertilization (IVF) and implantation
of 2-cell embryos in 1-3 pseudopregnant females to generate live pups
- This service does not include the price of donor females for IVF
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